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分子生物技术手册

English | 2024 | ISBN: 9781003055211 | 763 pages | True PDF | 30.25 MB

With a history that likely dates back to the dawn of human civilization more than 10,000 years ago, and a record that includes the domestication and selective breeding of plants and animals, the harnessing of fermentation process for bread, cheese, and brewage production, and the development of vaccines against infectious diseases, biotechnology has acquired a molecular focus during the 20th century, particularly following the resolution of DNA double helix in 1953, and the publication of DNA cloning protocol in 1973, and transformed our concepts and practices in disease diagnosis, treatment and prevention, pharmaceutical and industrial manufacturing, animal and plant industry, and food processing.

While molecular biotechnology offers unlimited opportunities for improving human health and well-being, animal welfare, agricultural innovation and environmental conservation, a dearth of high quality books that have the clarity of laboratory manuals without distractive procedural details and the thoroughness of well-conversed textbooks appears to dampen the enthusiasm of aspiring students. In attempt to fill this glaring gap, Handbook of Molecular Biotechnology includes four sections, with the first three presenting in-depth coverage on DNA, RNA and protein technologies, and the fourth highlighting their utility in biotechnology. Recognizing the importance of logical reasoning and experimental verification over direct observation and simple description in biotechnological research and development, the Introduction provides pertinent discussions on key strategies (i.e., be first, be better, and be different), effective thinking (lateral, parallel, causal, reverse, and random), and experimental execution, which have proven invaluable in helping advance research projects, evaluate and prepare research reports, and enhance other scientific endeavors.

Key features

Presents state-of-the-art reviews on DNA, RNA and protein technologies and their biotechnological applications Discusses key strategies, effective thinking, and experimental execution for scientific research and development Fills the gap left by detailed-ridden laboratory manuals and insight-lacking standard textbooks Includes expert contributions from international scientists at the forefront of molecular biotechnology research and development Written by international scientists at the forefront of molecular biotechnology research and development, chapters in this volume cover the histories, principles, and applications of individual techniques/technologies, and constitute stand-alone, yet interlinked lectures that strive to educate as well as to entertain. Besides providing an informative textbook for tertiary students in molecular biotechnology and related fields, this volume serves as an indispensable roadmap for novice scientists in their efforts to acquire innovative skills and establish solid track records in molecular biotechnology, and offers a contemporary reference for scholars, educators, and policymakers wishing to keep in touch with recent developments in molecular biotechnology.


拥有大约10000年前就可能追溯到的人类文明的悠久历史,以及包括植物和动物驯化与选择性培育、面包、奶酪和发酵饮品生产过程中发酵过程的应用,以及对抗病原体疾病开发疫苗在内的记录,生物技术在20世纪获得了分子焦点,特别是自1953年双螺旋DNA结构的解开和1973年DNA克隆方法的发表以来,这改变了我们在疾病诊断、治疗和预防方面、制药与工业制造、畜牧业和植物业以及食品加工方面的概念与实践。

虽然分子生物技术为改善人类健康福祉、动物福利、农业创新和环境保护提供了无限机会,但在高质量书籍缺乏,这些书籍既没有实验步骤的详细说明也没有学术著作中详尽的内容的情况下,似乎削弱了有志之士的热情。试图填补这一明显的空白,《分子生物学手册》包括四个部分,其中前三个部分涵盖了DNA、RNA和蛋白质技术的深入覆盖,第四部分则突出了它们在生物技术中的应用。

介绍部分提供了关于关键策略(例如:先行者、更好者和不同者)、有效思考(例如:横向思考、并行思考、因果关系、逆向思考和随机思维)以及实验执行的关键讨论,这些已被证明对于帮助推进研究项目、评估和准备研究报告以及增强其他科学活动非常有用。

关键特点

提供DNA、RNA和蛋白质技术及其生物技术应用的最新综合评论 探讨科学研究与开发中的关键策略、有效思考方法和实验执行 填补详细繁冗的实验室手册中缺乏洞见的标准教科书留下的空白 包括分子生物学研究前沿领域国际科学家的专家贡献 本卷章节覆盖个体技术/技术的历史、原理及其应用,构成既独立又相互关联的讲座,旨在教育并娱乐。除了作为分子生物学及相关领域大学三年级学生的知识性教科书之外,本卷还为希望在分子生物学方面获得创新技能和建立坚实记录的新手科学家提供一个不可或缺的地图,并成为希望了解最近分子生物学进展的学者、教师和政策制定者的一个当代参考。


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