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HIV协议,第四版

English | 2024 | ISBN: 1071638610 | 354 Pages | PDF (True) | 11 MB

This fourth edition volume expands on the previous editions with discussions on the latest methodologies to study HIV, live cell imaging, HIV cure, new modifications to the viral RNA that impacts HIV biology, and new types of intracellular compartments. The chapters in this book are organized into seven parts and cover topics such as HIV latency reactivation via single molecule RNA detection; T cell responses; new and efficacious anti-HIV CAR T cells; analysis of mucosal HIV infection; analysis of 3D brain organoids to study neuro AIDS; and the transfer of antibodies across the blood brain barrier. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


这本书的第四版在之前的版本上扩展,讨论了最新的方法来研究艾滋病病毒、活细胞成像、艾滋病治愈的新方法、对影响艾滋病生物学的病毒RNA的新修改以及新的细胞内囊泡类型。本书章节分为七部分,涵盖HIV潜伏性再激活通过单分子RNA检测;T细胞反应;新且有效的抗艾滋病CAR T细胞;分析黏膜内的艾滋病感染;用三维大脑器官样物来研究神经系统中的艾滋病;抗体跨过血脑屏障的转移等主题。以《Molecular Biology方法》系列成功的格式编写,每章包括其各自主题的简介、所需材料和试剂清单、步骤齐全且易于重复的实验室规程以及解决和避免已知陷阱的方法建议。
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