肝脏癌变：方法与协议

English | 2024 | ISBN-10: 1071636936 | 240 pages| Epub PDF (True) | 46 MB

This volume discusses the latest advancements in modern methodologies used to study liver carcinogenesis. The first half of this book describes pertinent preclinical models of hepatocellular carcinoma (HCC) and cholangiocarcinoma (CCA), established either through orthotopic induction of ectopic implantation. The second half of this book covers a diverse array of techniques applied to characterize the biochemical and cellular composition of hepatic malignancies that operate at the single-cell and histological levels. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

本书讨论了现代方法学在研究肝癌方面的最新进展。这本书的前半部分描述了通过原位诱导异位植入建立的相关肝细胞癌（HCC）和胆管癌（CCA）的预临床模型。这本书的后半部分涵盖了应用于表征肝脏恶性肿瘤的单细胞和组织水平上生化和细胞组成的各种多样化的技术。以《分子生物学方法》系列书籍的成功格式编写，章节包括各自主题的介绍、必要的材料和试剂清单、一步步易于重现的实验室规程以及解决遇到的问题并避开已知陷阱的小贴士。

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