兽医PCR诊断法

English | ISBN: 1608055728 | 2012 | PDF | 146 pages | 4 MB

PCR (Polymerase Chain Reaction) technology has become an indispensable component of routine veterinary diagnostics. However, a number of pitfalls and limiting factors affect its sensitivity and specificity of detection. It is imperative that veterinary PCR diagnosticians include such considerations in their work. Extensive experience with PCR technology in both research and diagnostic applications enables researchers to pinpoint these practical limitations, and therefore instruct the user in approaches that avoid these common errors. This book discusses the basic concepts, chemistries, and instrumentation of standard and real-time PCR, and includes present applications and future perspectives for veterinary diagnostics. Critical pitfalls have been highlighted in this text with regard to veterinary PCR diagnostics which include: i) choice of platform technologies for PCR diagnosis; ii) construction of optimized PCR primers and probes to ensure its highest specificity and sensitivity; iii) correct sampling and efficient methods to preserve and release nucleic acids; iv) maximization of PCR amplification efficiency; and v) avoidance and monitoring of contamination in Veterinary PCR Diagnostics. This Book should be a valuable reference for all veterinary professionals interested in this modern diagnostic tool.

PCR（聚合酶链反应）技术已成为常规兽医诊断中的不可或缺组成部分。然而，多种陷阱和限制因素影响其检测的敏感性和特异性。兽医PCR诊断员必须在工作中考虑这些因素。在研究和诊断应用中广泛使用PCR技术的经验使研究人员能够识别实际局限性，并因此指导用户避免这些常见的错误。本书讨论了标准PCR和实时PCR的基本概念、化学成分和技术仪器，以及兽医诊断的应用现状和发展前景。书中针对兽医PCR诊断的关键陷阱进行了突出说明，包括：i）选择用于PCR诊断的平台技术；ii）构建优化的PCR引物和探针以确保其最高的特异性和敏感性；iii）正确的样本采集及高效的保存和释放核酸的方法；iv）最大化PCR扩增效率；以及v）避免并监控兽医PCR诊断中的污染。这本书对于所有对这项现代诊断工具感兴趣的兽医专业人士来说，都应是一本宝贵的参考书。

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