使用MEMS技术进行全血的在芯片预处理

English | ISBN: 1608055280 | 2018 | 124 pages | PDF | 11 MB

The Microfabrication technology has stimulated a plurality of lab-on-a-chip research for biomedical researchers and health care practitioners to manipulate and analyze complex biological fluids at the nano and microliter scale. Specifically, various miniaturized diagnostic devices have been developed for blood sample detection, which can increase diagnostic capacity significantly by enabling rapid, point-of-care chemical analysis. Recently, more and more researchers are paying attention to on-chip whole blood sample pretreatment, which is the focus of this book, aiming to form real Micro Total Analysis Systems (uTAS) for whole blood analysis by integrating blood sample pre-treatment and detection together. The book consists of six chapters. Chapter 1 gives a brief introduction on the background of whole blood and the concept of µTAS, followed by a summary of specific microfluidic chips for whole blood pretreatment. Chapter 2 firstly reviews microfluidic chips for plasma isolation from whole blood samples with various methodologies (i.e., microfiltration, microcentrifugation, ultrasound, Zweifach–Fung effect and capillary effect). Then two examples using the plasma skimming effect and crossflow filtration respectively are discussed in detail. Chapter 3 firstly reviews microfluidic devices for blood cell filtrating, sorting and collection. Sorting methods such as size filtration, optical and magnetic manipulation, affinity separation, dielectrophoretic manipulation are covered and compared. Then a case study using crossflow filtration is analyzed in detail. Chapter 4 firstly reviews reported microfluidic devices for blood cell lysis in which mechanical lysis, sonication lysis, thermal lysis, electrical lysis, chemical lysis and optical lysis are covered. And then a cell lysis microchip using chemical agents for cell lysis is chosen as an example for further concept demonstration. Chapter 5 firstly reviews microfluidic devices for DNA extraction and purification by comparing reported methods of solid phase extraction, solid phase reversible immobilization and liquid/liquid extraction. And then two microchips with different solid phase matrixes are used for demonstration of chip based DNA extraction. Chapter 6 firstly reviews current integrated microfluidic platforms for blood pretreatment, followed by a case study. Both fundamental analysis and specific applications are presented in this book, which is written for researchers, engineers, and advanced students who have research interest in whole blood pretreatment and miniaturized techniques. We would like to express our appreciation to Bentham Science Publishers and their team members.

微fabrication技术激发了生物医学研究者和医疗保健从业者进行复杂的生物液体操纵与分析的lab-on-a-chip研究。特别是，开发了一系列微型诊断设备用于血液样本检测，这些设备可以通过使快速、现场化学分析成为可能来显著增加诊断容量。最近越来越多的研究人员关注芯片上的全血样本预处理技术，这是本书的重点目标，旨在通过将血样预处理和检测整合在一起，从而形成真正的micro Total Analysis Systems（uTAS）用于全血分析。书中共有六章内容。第1章简要介绍了全血的基本背景和µTAS的概念，并总结了用于全血预处理的微流控芯片。第2章首先回顾了全血样本中分离血浆的各种方法（包括微过滤、微离心、超声波技术、Zweifel–Fung效应和毛细作用）。然后，详细讨论了两种使用血浆抽吸效果和交叉流动过滤的不同实例。第3章首先回顾了用于血液细胞过滤、排序与收集的微流控设备。大小过滤法、光学控制、磁力操控、亲和分离以及电场操控被涵盖并比较。随后详细分析了一个使用交叉流动过滤的例子。第4章首先回顾了用于全血细胞溶解的各种微流控装置，涵盖了机械性溶解放置、超声波性溶解放置、热解离、电气性溶解放置、化学性溶解放置和光学性溶解放置方法。然后选择一个使用化学试剂进行细胞溶解的细胞溶解芯片作为进一步概念展示的例子。第5章首先回顾了用于DNA提取与纯化的微流控装置，通过比较已报告的方法：固体相萃取法、可逆固态结合固定化技术和液-液萃取技术来介绍这些方法。然后使用两种具有不同固体相基质的芯片进行基于芯片的DNA提取演示。第6章首先回顾了当前用于全血预处理的集成微流控平台，随后进行了一个案例研究。本书为对全血预处理和微型化技术感兴趣的研究人员、工程师和高级学生提供了基本分析与具体应用。我们衷心感谢Bentham Science Publishers及其团队成员的支持。

---

Download from free file storage

 Download from nitroflare.com  Download from rapidgator.net

---

我要反馈

---

本站不对文件进行储存，仅提供文件链接，请自行下载，本站不对文件内容负责，请自行判断文件是否安全，如发现文件有侵权行为，请联系管理员删除。